In this chapter you have learned how to draw regions of interest over your image, save them for later use, and perform local analysis. Also, the filtering concept has been introduced along with several useful applications. Finally, we have shown how to combine several tools in order to clean an image and perform a particle analysis over it.

So far, we have been doing everything manually. In the next chapter, we will introduce the ImageJ macro system, which will allow you to repeat exactly the same operations on a greater number of images, or to save exactly the processing steps you carry out to ensure repeatability of your measurements.